Flag antibody proteintech
WebApr 8, 2024 · The antibodies used in IHC analysis are listed in Supplementary Table S4. ... E. Coli derived GST-Vector or GST-PRDX1 fusion protein was purified and prepared by Proteintech (Wuhan, China). Briefly, the total protein of 293T cells with or without Flag-tagged IRAK1 overexpression was incubated with GST-PRDX1 or GST-Vector fusion …
Flag antibody proteintech
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WebThe proteins retained on beads were subjected to immunoblot using anti-GST and anti-Flag antibodies. F 293T cells were transfected with si-Control or si-p62 under MG132 treatment. ... (1:5000; SA00001-1), and goat polyclonal anti-rabbit IgG (1:5000; SA00001-2) were obtained from Proteintech; antibody against pan–acetyl-lysine (1:2000, 9441S ... WebApr 6, 2024 · Cells were then incubated with primary antibody for 2 h at RT, followed by incubation with secondary antibody for 1 h. A wash step with 0.1% PBS-0.1%Tween (pH = 7.5) was applied after each incubation step. Finally, the cells were imaged under a confocal microscope. Proteomics. Flag bead-tagged proteins were loaded and run on …
WebApr 10, 2024 · Mouse monoclonal anti-GAPDH Antibody: Proteintech: Cat# 60004-1-Ig; RRID: AB_2107436: Rabbit monoclonal anti-APP Antibody (Y188) ... Mouse monoclonal anti-FLAG Antibody (clone M2) SIGMA: Cat# F1804; RRID: AB_262044: Mouse monoclonal anti-V5 Antibody: ABclonal: Cat# AE017; RRID: AB_2770413: Rabbit … WebThe 3xFLAG ® system is an improvement upon the original system by fusing three tandem FLAG ® epitopes for a total of 22 amino acids (Figure 1).Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. Like the original FLAG tag, 3xFLAG ® is hydrophilic, contains an Enterokinase cleavage site, and …
WebProduct Description. This is a polyclonal rabbit anti-FLAG antibody labeled with our superior CF® Dyes. This antibody recognizes the FLAG epitope tag (DYKDDDDK) … WebProteintech Group, Inc.（プロテインテック）社は、2001年に設立された抗体製造メーカーです。 現在までに、米国本社の他、英国、中国、ドイツ、フランス、シンガポール等で支社を展開し、各国で様々なマーカー抗体、タンパク質研究用試薬を供給しています。
WebDYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N …
WebProteintech提供覆盖人类基因组靶标的经过可重复性验证的抗体。 抗体 免疫分析 一抗 FlexAble抗体标记试剂盒 New 直标抗体 更多 ELISA试剂盒 IHCeasy即用型试剂盒 细胞 … designer birthday cakes in gurgaonWebMouse Anti Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … chubby exhaustWebThese antibodies have been verified by Relative expression to confirm specificity to HA Tag. Find the HA Tag antibody that fits your needs. Choose from 1 of 92 HA Tag antibodies, which have been validated in experiments with 527 publications and 249 images featured in our data gallery. Browse primary antibodies for WB, Flow, IHC, … designer birthday cakes raleigh ncWebFeb 2, 2024 · Flag IP was performed and analyzed by western blotting with Flag or myc antibodies. (C) N-TAD domain of AR interacted with SMAD3. Myc-SMAD3 was co-expressed with the Flag-tagged AR fragments (N-terminal transactivation domain, N-TAD; DNA binding domain, DBD; Ligand-binding domain, LBD) in 293T cells, and analyzed as … chubby english comedianWebApr 6, 2024 · Long-term memory formation requires de novo RNA and protein synthesis. By using the differential display-polymerase chain reaction strategy, we have presently identified the Nedd4 family interacting protein 1 (Ndfip1) cDNA fragment that is differentially expressed between the slow learners and the fast learners from the water maze learning … designer birthday party decorationsWebFor in vitro co-immunoprecipitation (co-IP) analysis, FLAG-tagged proteins were produced in HTR-8/Svneo and JAR cells transfected with LV-PADI6. Total cell lysates were incubated overnight at 4°C with anti-YAP1 antibodies (Proteintech, Wuhan, China), anti-FLAG antibodies (Sigma, MO, USA), or normal IgG (Beyotime, Shanghai, China) as a control. designer birthday cakes with circlesWebThe primary antibodies against α-actinin (# 11313-2-AP) and Flag (DYKDDDDK) tag (# 20543-1-AP) were bought in Proteintech Group (Chicago, IL, USA). CCL4 (# ab45690) and ADAMTS5 (# ab41037) were obtained. (12) SIRT2 promotes the viability, invasion and metastasis of osteosarcoma cells by inhibiting the degradation of Snail Cell Death Dis ... designer birthday cake ideas